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The method for classifying the thyroid status will be accomplished using state-of-the-art assay methodology.
Free T4 (FT4)—this procedure is considered to be the "gold standard" for assessment of thyroid's production and cellular availability of thyroxine. FT4 concentration is expected to be decreased in dogs with thyroid dysfunction due to autoimmune thyroiditis.
Canine thyroid simulating hormone (cTSH)—this procedure helps determine the site of the lesion in cases of hypothyroidism. In autoimmune thyroiditis the lesion is at the level of the thyroid gland and the pituitary gland functions normally. The cTSH concentration is expected to be abnormally elevated in dogs with thyroid atrophy from autoimmune thyroiditis.
Thyroglobulin Autoantibodies (TgAA)—this procedure is an indication for the presence of the autoimmune process in the dog’s thyroid.
Positive autoimmune thyroiditis
Positive compensative autoimmune thyroiditis
Idiopathically reduced thyroid function
All other results are considered equivocal